Abstract During studies on fish parasites, two new species of Anacanthorus were found parasitizing serrasalmid fishes, Anacanthorus simpliciphallus sp. n. from the hybrid Piaractus mesopotamicus x Piaractus brachypomus and Anacanthorus brandtii sp. n. from Serrasalmus brandtii. Anacanthorus simpliciphallus sp. n. resembles Anacanthorus reginae in the morphology of the male copulatory organ (MCO) and accessory piece but differs from A. reginae in terms of the smaller size of the accessory piece, which corresponds approximately half the size of the MCO and by the presence of a conspicuous metraterm, with a membranous terminal region. Anacanthorus brandtii sp. n. differs from Anacanthorus scapanus by the expansion of the accessory piece, from Anacanthorus jegui by the ratio MCO (male copulatory organ) /AP (accessory piece) and by the expansion of hook shank, from Anacanthorus sciponophallus and A. reginae by the ratio MCO/AP. Anacanthorus brandtii sp. n. can be distinguished from A. reginae and A. simpliciphallus sp.n. by the size of hooks which is similar in A. reginae and A. simpliciphallus sp. n. and dissimilar in A. brandtii sp. n. The two new species also differ from each other by the expansion of shank.

Resumo Durante estudos em parasitos de peixes, duas novas espécies de Anacanthorus foram encontradas parasitando peixes serrasalmideos, Anacanthorus simpliciphallus sp. n. do híbrido Piaractus mesopotamicus x Piaractus brachypomus e Anacanthorus brandtii sp. n. de Serrasalmus brandtii. Anacanthorus simpliciphallus sp. n. assemelha-se a Anacanthorus reginae na morfologia do órgão copulatório masculino (OCM) e peça acessória (PA), mas difere de A. reginae pelo tamanho menor da peça acessória, que corresponde a aproximadamente metade do tamanho do OCM e pela presença de um metraterma conspícuo, com uma região terminal membranosa. Anacanthorus brandtii sp. n. difere de Anacanthorus scapanus pela expansão da peça acessória, de Anacanthorus jegui pela proporção OCM / PA e pela expansão da haste do gancho, e de Anacanthorus sciponophallus e A. reginae pela proporção OCM / PA. Anacanthorus brandtii sp. n. pode ser distinguido de A. reginae e A. simpliciphallus sp. n. pelo tamanho dos ganchos que é similar em A. reginae e A. simpliciphallus sp. n. e dissimilar em A. brandtii sp. n. As duas espécies novas também diferem entre si pela expansão da haste do gancho.

ABSTRACT Salinity is one of the abiotic stresses that affect gas exchange and growth of pomegranate. In this context, the application of nitrogen fertilizer through the leaves can minimize these effects. Thus, the objective of present study was to evaluate the effect of foliar nitrogen fertilization on pomegranate seedlings irrigated with brackish water. The design used was randomized blocks, in an incomplete factorial scheme (Central Composite Design) with five electrical conductivities of irrigation water - ECw (0.50, 1.15, 2.75, 4.35, and 5.00 dS m-1) and five doses of foliar nitrogen fertilization - FNF (0, 0.33, 1.15, 1.97, and 2.30 g L-1), with four replicates and two plants per experimental plot. Plant height, number of leaves, stem diameter, and gas exchange (stomatal conductance, net photosynthesis, intercellular CO2 concentration, transpiration rate, instantaneous carboxylation efficiency, instantaneous water use efficiency, and intrinsic water use efficiency) were evaluated at 60 days after the beginning of irrigation with saline water. Salinity of irrigation water negatively affects the gas exchange of pomegranate seedlings. Foliar nitrogen fertilization up to 1.31 g L-1 improves plant height and number of leaves in pomegranate seedlings under salt stress. Foliar nitrogen fertilization up to 1.59 g L-1 increases the net photosynthesis of pomegranate seedlings.

RESUMO A salinidade é um dos estresses abióticos que afeta as trocas gasosas e o crescimento de romãzeira. Neste sentido, à aplicação de adubo nitrogenado via foliar poderá minimizar estes efeitos. Com isso, o objetivo do presente estudo foi avaliar o efeito da aplicação foliar com nitrogênio em mudas de romã irrigadas com águas salobra. O delineamento utilizado foi de blocos casualizados, em esquema fatorial incompleto (Composto Central de Box), sendo cinco condutividade elétrica da água - CEa (0,50; 1,15; 2,75; 4,35 e 5,00 dS m-1) e cinco doses de adubação nitrogenada via foliar - ANF (0; 0,33; 1,15; 1,97 e 2,30 g L-1), com quatro repetições. A salinidade da água de irrigação afeta negativamente as trocas gasosas de mudas de romãzeira. A adubação nitrogenada foliar até 1,31 g L-1 melhora o crescimento em altura e no número de folhas em mudas de romãzeira sob estresse salino. Adubação nitrogenada foliar até a dose de 1,59 g L-1 aumenta a fotossíntese das mudas de romãzeira.

ABSTRACT Purpose: Sling as a therapeutic option for male stress urinary incontinence (SUI) has been reviewed in the last two decades, as it is a relatively simpliest surgery compared to artificial urinary sphincter and has the ability to modulate urethral compression. This study aims to evaluate the efficacy, rate of complications, quality of life and the effects on bladder emptying of the Argus T® compressive and ajustable sling in moderate and severe male SUI treatment. Materials and Methods: Men eligible for stress urinary incontinence treatment after radical prostatectomy were recruited and prospectively evaluated, from March 2010 to November 2016. It was selected outpatient men with moderate and severe SUI, after 12 months of radical prostatectomy, who have failed conservative treatment. All patients had a complete clinical and urodynamic pre and post treatment evaluation, by means of clinical history, physical examination, urine culture, 1-hour pad test and ICIq-SF questionnaire. The UDS was performed after 12, 18 and 24 months postoperatively. Results: Thirty-seven men underwent sling surgery, 19 patients (51.4%) with moderate and 18 (48.6%) with severe SUI. The minimum follow-up time was 5 years. Overall, we had a success rate of 56.7% at 60 months follow-up. After surgery, we did not observe significant changes in the urodynamic parameters evaluated during the follow-up. No patient had urodynamic bladder outlet obstruction (BOO) after sling implantation. Readjustment of the Argus T® sling was performed in 16 (41%) of the patients and 51% of the patients reported some adverse event. Conclusion: We demonstrate a long-term efficacy and safety of Sling Argus T® as an alternative to moderate and severe male SUI treatment. Furthermore, in our study bulbar urethra compression does not lead to bladder outlet obstruction.

RESUMO Um dos processos mais usados para garantir a qualidade dos grãos em grandes armazéns é a aeração. Um modelo de otimização que considere a natureza limitada e discreta das entradas de ar, além das características geométricas do armazém dos grãos, pode produzir resultados mais facilmente aplicáveis no design de novos armazéns graneleiros. O objetivo deste estudo foi parametrizar e aplicar o modelo de Darcy-Forchheimer para simulação de fluxo de ar em massa de grãos de soja em função da altura da camada de grãos e desenvolver um método de inteligência artificial baseado em algoritmos genéticos para otimizar as dimensões do armazém de grãos e a configuração das entradas de ar para obter um fluxo de ar mais homogêneo na massa de grãos. A parametrização considerou o efeito da compactação dos grãos e as simulações em OpenFOAM mostraram boa concordância com os dados experimentais. O algoritmo genético proposto foi capaz de aumentar a homogeneidade do fluxo de ar se comparada ao armazém usado como referência. aeração graneleiros DarcyForchheimer Darcy Forchheimer experimentais referência

ABSTRACT One of the main processes used to maintain grain quality in large storage bins is aeration. An optimization model that considers the limited and discrete nature of the air inlets, in addition to the geometric characteristics of the storage bin, could produce results more easily applicable results for the design of new grain storage bins. The objective of this study was to parameterize and to apply the Darcy-Forchheimer model for simulating airflow in soybean grain mass as function of grain layer height, and develop an artificial intelligence method based on genetic algorithm for optimizing grain storage bin dimensions and air inlet configurations to obtain a more homogeneous airflow in the grain mass. The parameterization considered the effect of grain compaction and the OpenFOAM simulations showed good agreement with the experimental data. The proposed genetic algorithm was able to increase the airflow homogenization when compared to the grain storage bin used as reference. aeration inlets DarcyForchheimer Darcy Forchheimer height data reference

ABSTRACT Growers have adopted monoculture to maintain the high melon (Cucumis melo L.) production demand in the Northeastern region of Brazil. This cultivating practice culminates in up to three crop cycles per year being used in the same growing area. The main objective of this study was to evaluate if the incorporation of plant material used with polyethylene mulch and or in association with commercial soil amendment products can help to condition an environment that is beneficial to soil microbial communities. Two identical greenhouse experiments were conducted using a completely randomized design with seven treatments and seven replications. The treatments were: (C) – Control, (M) - polyethylene mulch, (C+M) - incorporation of Crotalaria juncea L. + polyethylene mulch, (P+M) - incorporation of Pennisetum glaucum L. + polyethylene mulch, (M+CS) - polyethylene mulch + (Compost-Aid® + Soil-Set®, trade names of products produced by Alltech Crop Science), (C+M+CS) - incorporation of C. juncea L. + polyethylene mulch + (Compost-Aid® + Soil-Set®), and (P+M+CS) - incorporation of P. glaucum L. + polyethylene mulch + (Compost-Aid® + Soil-Set®). To quantify the target soil microbiota (fungi, bacteria, sporulating bacteria, and actinomycetes), isolations were attempted on selective culture media specific for each group of microorganisms. The incorporation of P. glaucum together with the use of polyethylene mulch and commercial products (Compost-Aid® and Soil-Set®), (P+M+CS), increased the total fungal population by 183%, total bacteria by 55%, sporulating bacteria by 21%, and actinomycetes by 146% in relation to the control treatment.

RESUMO Os produtores há anos adotam a monocultura para manter a demanda de produção de melão alto (Cucumis melo L.) na região Nordeste do Brasil. Esta estratégia de cultivo é usada em até três ciclos por ano. O objetivo principal deste estudo foi avaliar se a incorporação de material vegetal utilizado com mulch de polietileno e/ou em associação com produtos corretivos de solo pode condicionar um ambiente benéfico para as comunidades microbianas do solo. Dois experimentos idênticos em casa de vegetação foram conduzidos em um delineamento inteiramente casualizado com sete tratamentos e sete repetições. Os tratamentos foram: (C) – Controle, (M) - cobertura de polietileno, (C+M) - incorporação de Crotalaria juncea L. + cobertura de polietileno, (P+M) - incorporação de Pennisetum glaucum L. + cobertura de polietileno, (M+CS) - cobertura de polietileno + (Compost-Aid® + Soil-Set®, nomes comerciais dos produtos produzidos pela Alltech Crop Science), (C+M+CS) - incorporação de C. juncea L. + cobertura de polietileno + (Compost-Aid® + Soil-Set®) e (P+M+CS) - incorporação de P. glaucum L. + cobertura de polietileno + (Compost-Aid® + Soil-Set®). Para quantificar a microbiota do solo (fungos, bactérias, bactérias esporulantes e actinomicetos), foram realizados isolamentos em meios de cultura seletivos para cada grupo de microrganismos. Conclui-se que, coletivamente, a incorporação de P. glaucum juntamente com o uso de mulch de polietileno e os produtos comerciais (Compost-Aid® e Soil-Set®), aumentou a população total de fungos em 183%, bactérias totais em 55%, bactérias esporulantes em 21% e actinomicetos em 146% em relação ao tratamento controle.

Tucumã (Astrocaryum aculeatum) pulp presents a large number of secondary metabolites that can mediate the synthesis of metallic nanoparticles through a greener and more sustainable synthetic route than traditional ones. This study investigated the production of silver nanoparticles (AgNPs) mediated by aqueous extracts of tucumã pulp assessing different parameters, such as temperature, concentration of extract, and pH values of the reaction media. The alkaline reaction media produced the most intense and well-defined bands around 400 nm in the electronic spectra, indicating the formation of AgNPs in the three concentrations of tucumã pulp evaluated. The increase in the temperature of the reaction media also enhanced the surface plasmon resonance band of AgNPs but it increased the polydispersivity of produced nanoparticles according to the dynamic light scattering (DLS) and transmission electron microscopy (TEM) data. Zeta potential results indicated moderate stability of the AgNPs (close to -30 mV). Antimicrobial assessment of the colloidal metal particles indicated microbicide activity against the Gram-negative Escherichia coli bacterium.

Abstract Objective: To investigate the effects of different physical exercise programs and polymorphisms of the FTO (fat mass and obesity-associated gene) on body composition and cardiovascular risk factors in adolescents with overweight and obesity. Methods: A randomized, parallel, double-blind clinical trial consisting of the adolescent overweight from the state public network, in a simple representative random sample, who participated in an aerobic exercise or weight training intervention for 10 weeks. Anthropometry, body composition, biochemical markers, sexual maturation, and rs9939609 polymorphism in the FTO gene were assessed. 347 adolescents had their characterization of nutritional status. 72 individuals with overweight and obesity were invited to participate. 39 remained for the start of the program and were randomly allocated to both types of intervention. In the end, 26 subjects participated in the intervention programs, with 12 and 14 in the aerobic and weight training programs, respectively. Results: Heterozygous and homozygous bearers of risk allele A participating in the aerobic program showed improvements in glycemia (p = 0.002) and total cholesterol (p = 0.023) and a reduction in body fat mass (p = 0.041). The weight training program reduced glycemia in patients with the risk allele A (p = 0.027). Cameron’s stage four sexual maturation participants were 2.1 times more likely to improve their body fat (CI = 1.31–3.39). Conclusion: Aerobic exercises produced exclusively a significant decrease in fat mass and total cholesterol in patients with risk allele A. Distinct physical exercise programs may cause diverse changes in risk variables related to the health of adolescents.

Abstract This study evaluated the bioavailability of polycyclic aromatic hydrocarbons (PAHs) in Santos Bay (SB) and the adjacent Santos Continental Shelf (SCS) in Brazil. Biliary metabolites were measured in several fish species to establish a baseline for future monitoring programs. Bile samples from different species of fish were collected monthly from July to December 2005 in SB, and in August 2005 and February 2006 on SCS. Metabolite concentrations were determined using high-performance liquid chromatography with fluorescence detectors. Naphthalene, phenanthrene, and benzo[a]pyrene metabolite concentrations ranged from 24 to 810 µg g -1 of bile, 1.8 to 68 µg g -1 of bile, and below the limit of quantitation to 1.3 µg g -1 of bile, respectively. Despite its high concentrations, the levels of naphthalene metabolites were in regions of low-contamination, while benzo[a]pyrene metabolite were in the same range as those reported in moderately contaminated areas, which may indicate pyrolytic contamination by PAHs. No significant differences in the metabolite concentrations were found between the SB and the SCS samples or during the periods of collection. Future studies with a single biomonitoring species should be conducted, considering age, sex, and feeding condition of the individuals. The metabolite data presented in this study is an important baseline information for this urbanized region, which hosts several sources of contaminants.

Abstract This study was conducted to examine the effects of a diet formulated with industrial amino acids and a commercial vitamin-mineral mixture on the characteristics of carcass and meat cuts of slow-growing broilers slaughtered at different ages. The experiment involved 600 one-day-old male and female white Naked Neck chicks. The chicks were allotted randomly to a 2 × 3 factorial arrangement consisting of two diets and three slaughter ages, in a completely randomized experimental design with five replicates of 20 birds each. The experimental period was 84 days. Six chicks were selected and slaughtered on the first day, and then at 56, 70 and 84 days of age 10 birds unit was selected and slaughtered per experimental period. Weight and yield of carcass, abdominal fat and breast, thigh and drumstick meat were determined. Additionally, moisture, protein, fat and mineral matter contents and protein and fat deposition in the breast, drumstick and thigh muscles were determined. The diet did not influence the yields of carcass, abdominal fat, or meat cuts or the nutritional composition of meat. Slaughter age influenced the yields of breast and thigh meat and abdominal fat; the protein content of breast meat; and the moisture, crude protein, fat, and mineral matter contents of drumstick and thigh meat. There was an interaction effect between diet and slaughter age for protein deposition in breast meat. The age factor influenced fat deposition in the drumstick muscles. Protein deposition in the thigh muscles was influenced by the diet. Moreover, a difference was observed between the ages for protein and fat deposition in the thigh muscle. In conclusion, the use of a commercial mixture in the diet results in decreased protein deposition in the breast and thigh muscles, although this difference does not affect the characteristics of carcass or meat cuts. Birds slaughtered at 70 days exhibit similar carcass characteristics to those of birds slaughtered at 84 days, indicating the possibility of an earlier slaughter age.

RESUMO O estudo visa quantificar os custos de produção das culturas de milho e sorgo em condições de sequeiro e irrigado para a geração de etanol, e avaliar a viabilidade econômica em diferentes cenários de preço de comercialização. Foram realizados dois experimentos nos anos de 2019/2020 e 2020/2021, em área experimental da Universidade Federal de Santa Maria, Santa Maria, RS. O delineamento experimental foi em faixas, com blocos ao acaso e quatro repetições. Foram avaliadas três lâminas de irrigação: 0, 50 e 100% da evapotranspiração de referência (ETo) como o primeiro fator, e duas cultivares de milho e uma de sorgo granífero como o segundo fator, sendo utilizados 45 cenários. O uso da irrigação plena (100% de ETo) reduz os custos de produção do litro de etanol em 15 e 17,89% e aumenta a produção de etanol em 44,18 e 48,25% para as culturas de milho e sorgo, respectivamente. Para o preço de mercado de R$ 2,00 L-1, o sorgo granífero não apresenta bom desempenho, com valor presente líquido (NPV) e taxa interna de retorno (IRR) negativos. O preço de venda de R$ 4,00 L-1 e irrigação plena é o melhor cenário econômico, com valores de NPV de R$ 90.356,93, IRR de 33,83%, relação benefício/custo (B/C) de 2,28, índice de rentabilidade (PI) de 3,99 e payback de três anos. O milho é economicamente viável para a produção de etanol em todos os cenários, sendo a irrigação plena e preço de comercialização de R$ 4,00 L-1, o que apresentou o melhor cenário econômico, com valores médios para o NPV de R$ 204.381,68, IRR de 63,35%, B/C de 2,96, IR 8,67 e payback de 1,58 anos, entre as cultivares.

ABSTRACT The study aims to quantify the production costs of maize and sorghum crops under rainfed and irrigated conditions for the generation of ethanol and to evaluate the economic feasibility in different scenarios of marketing price. Two experiments were carried out in the years 2019/2020 and 2020/2021, in an experimental area at the Federal University of Santa Maria, Santa Maria, RS, Brazil. The experiment was in strip plots, in a randomized block design and four replicates. Three irrigation depths, 0, 50 and 100% of the reference evapotranspiration (ETo), as first factor, and two maize cultivars and one grain sorghum cultivar as second factor, using 45 scenarios. The use of full irrigation (100% of ETo) reduces the production costs of the liter of ethanol by 15 and 17.89% and increases ethanol production by 44.18 and 48.25% for maize and grain sorghum crops, respectively. For the market price of R$ 2.00 L-1, the grain sorghum does not show good performance, with negative net present value (NPV) and internal rate of return (IRR). The sale price of R$ 4.00 L-1 and full irrigation is the best economic scenario, with values of NPV of R$ 90,356.93, IRR of 33.83%, benefit/cost ratio (B/C) of 2.28, profitability index (PI) of 3.99 and payback of three years. Maize is economically viable for ethanol production in all scenarios. Full irrigation and market price of R$ 4.00 L-1 represent the best economic scenario, with mean values for NPV of R$ 204,381.68, IRR of 63.35%, B/C of 2.96, PI of 8.67 and payback of 1.58 years, among the cultivars.

ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.

Abstract Providencia stuartii is one of the Enterobacteriaceae species of medical importance commonly associated with urinary infections, which can also cause other ones, including uncommon ones, such as liver abscess and septic vasculitis. This bacterium stands out in the expression of intrinsic and acquired resistance to antimicrobials. Besides, it uses mechanisms such as biofilm for its persistence in biotic and abiotic environments. This study investigated the cellular hydrophobicity profile of clinical isolates of P. stuartii. It also analyzed genes related to the fimbrial adhesin in this species comparing with other reports described for other bacteria from Enterobacteriaceae family. The investigated isolates to form biofilm and had a practically hydrophilic cell surface profile. However, fimH and mrkD genes were not found in P. stuartii, unlike observed in other species of Enterobacteriaceae. These results show that P. stuartii has specificities regarding its potential for biofilm formation, which makes it difficult to destabilize the infectious process and increases the permanence of this pathogen in hospital units.

Resumen Antecedentes: Las complicaciones tras la colangiopancreatografía retrógrada endoscópica (CPRE) que necesitan manejo quirúrgico son limitadas y aproximadamente el 0.09-1.8% corresponden a perforación duodenal. Este tipo de lesiones requieren un diagnóstico temprano y un manejo adecuado, ya que los desenlaces pueden ser catastróficos, con una mortalidad reportada de hasta el 20%. Objetivo: Presentar nuestra experiencia en el manejo quirúrgico de estas lesiones. Método: Estudio observacional, retrospectivo y descriptivo, de pacientes que requirieron manejo quirúrgico por perforación duodenal tras CPRE en tres centros de Bogotá, Colombia, entre enero de 2013 y diciembre de 2018. Resultados: Fueron llevados a manejo quirúrgico 13 pacientes, 7 (53.8%) por vía laparoscópica, con una estancia hospitalaria promedio de 10.7 días y una mortalidad del 0%, y 6 (46.1%) por vía abierta, con una estancia hospitalaria promedio de 33 días y una mortalidad del 50%, con seguimiento mínimo a 6 meses. Conclusiones: La perforación duodenal tras una CPRE, pese a su baja incidencia, se debe sospechar por su alta morbimortalidad. La decisión del abordaje quirúrgico está condicionada por el estado clínico del paciente, y por ende está ligada al pronóstico de este.

Abstract Background: Complications after endoscopic retrograde cholangiopancreatography (ERCP) are rare, approximately 0.09% to 1.8% are duodenal perforation. This type of injury requires early diagnosis and proper management since the outcomes can be catastrophic, leading to death in 20% of cases. Objective: To show our experience in the management of these injuries in order to establish if there is benefit from minimally invasive management versus conventional management. Method: Observational, retrospective and descriptive study comparing patients who required surgical management after post-ERCP duodenal perforation in three centers in Bogotá, Colombia, between January 2013 and December 2018. Results: 13 patients were taken to surgical management after duodenal perforation post ERCP, 7 laparoscopically (53.8%), with an average hospital stay of 10.7 days and mortality of 0% and 6 by open route (46.1%), with an average hospital stay of 33 days and mortality of 50%, with a minimum follow-up of 6 months. Conclusions: Post ERCP duodenal perforation, despite its low incidence, should be suspected due to its high mortality. Laparoscopic surgery is a good tool to address duodenal perforations after ERCP, because it presents a lower rate of complications and mortality.

CHEMICAL COMPOSITION AND PHOTOPROTECTIVE AND ANTIRADICAL ACTIVITIES OF THE BRANCHES OF Platonia insignis (CLUSIACEAE). The chemical study of the branches of Platonia insignis enabled the isolation of the biflavonoid morelloflavone and the identification, by mass spectrometry, of over 9 polyphenols, including eight biflavonoids, one benzophenone, and one xanthone. The in vitro biological tests of solar photoprotection and antiradical activities against the crude extract and Hex and AcOEt fractions showed relevant results, which confirm the high pharmaceutical potential of the species.

Leishmaniasis, a neglected tropical disease with a high worldwide incidence, is considered a public health issue in Minas Gerais and Brazil, with a high degree of morbidity, not to mention the lack of therapeutic arsenal. The cysteine protease (rCPB2.8) and cyclin dependent kinase (CRK3), important enzymes for the parasite’s feasibility, were the targets chosen for investigation of the new drugs. The following study aimed to analyze several oximic derivatives starting from safrol, which can present an affinity profile for selected molecular targets using tools from molecular modeling and bioinformatics, planning and synthesis of brand new substances being tested for leishmanicidal drugs. The study allowed to verify that three oximic derivatives (5a, 5f and 5h) presented high affinity for the CRK3 enzyme, and that the compounds 5c and 5g presented good interaction by the amino acids of the catalytic site of the rCPB2.8 enzyme with atomic distances capable of generating covalent bonds, which are essential for enzyme inhibitory activity.